Detection of Enterotoxigenic Potential of <i>Staphylococcus Aureus</i> Isolates from Cheese Samples with Two Different Methods

Abstract The primary objective of our study was to detect the occurrence enterotoxigenic Staphylococcus aureus in diverse types cheese (cow’s milk and mixed cheese) samples from R.N. Macedonia. Cheese were analyzed for enumeration isolation S. strains according ISO 6888-1. We detected toxigenic potential by use Enzyme Link Fluorescent Assay VIDAS system, we confirmed presence SEs ( sea, seb, sec, sed, see ) genes multiplex PCR. results showed that out 270 cheese, coagulase-positive staphylococci (CPS) 27 (10%), coagulase-negative five (1.8%). Biochemically, all CPS be aureus. With SET2 test 11 isolates are producers one toxins limited test. conventional PCR only 7 isolates. Most common gene seb n=3 (42.8%), followed sea n=2 (28.6%), sec (28.6%). Additionally, sed not any Discrepancies between two methods detection uncommon. viable cells have enterotoxin potency demonstrates importance appropriate hygiene practices diary process also maintenance products order obtain a safe final product consumers.